A discussion of a new study from scientists in Sweden.

Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line

Preclinical studies of COVID-19 mRNA vaccine BNT162b2, developed by Pfizer and BioNTech, showed reversible hepatic effects in animals that received the BNT162b2 injection. Furthermore, a recent study showed that SARS-CoV-2 RNA can be reverse-transcribed and integrated into the genome of human cells. In this study, we investigated the effect of BNT162b2 on the human liver cell line Huh7 in vitro. Huh7 cells were exposed to BNT162b2, and quantitative PCR was performed on RNA extracted from the cells. We detected high levels of BNT162b2 in Huh7 cells and changes in gene expression of long interspersed nuclear element-1 (LINE-1), which is an endogenous reverse transcriptase. Immunohistochemistry using antibody binding to LINE-1 open reading frame-1 RNA-binding protein (ORFp1) on Huh7 cells treated with BNT162b2 indicated increased nucleus distribution of LINE-1. PCR on genomic DNA of Huh7 cells exposed to BNT162b2 amplified the DNA sequence unique to BNT162b2. Our results indicate a fast up-take of BNT162b2 into human liver cell line Huh7, leading to changes in LINE-1 expression and distribution. We also show that BNT162b2 mRNA is reverse transcribed intracellularly into DNA in as fast as 6 h upon BNT162b2 exposure.
(https://www.mdpi.com/1467-3045/44/3/73/htm)

(credit: High Intensity Health)
𝘈𝘭𝘭 𝘤𝘳𝘦𝘥𝘪𝘵 𝘨𝘰𝘦𝘴 𝘵𝘰 𝘤𝘰𝘱𝘺𝘳𝘪𝘨𝘩𝘵 𝘩𝘰𝘭𝘥𝘦𝘳(𝘴).*
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NOTE: 𝘛𝘩𝘦 𝘪𝘯𝘧𝘰𝘳𝘮𝘢𝘵𝘪𝘰𝘯, 𝘪𝘥𝘦𝘢𝘴, 𝘢𝘯𝘥 𝘷𝘪𝘦𝘸𝘴 𝘪𝘯 𝘵𝘩𝘪𝘴 𝘤𝘰𝘯𝘵𝘦𝘯𝘵 𝘢𝘳𝘦 𝘵𝘩𝘰𝘴𝘦 𝘰𝘧 𝘵𝘩𝘦 𝘢𝘶𝘵𝘩𝘰𝘳(𝘴) / 𝘴𝘱𝘦𝘢𝘬𝘦𝘳(𝘴) / 𝘱𝘳𝘦𝘴𝘦𝘯𝘵𝘦𝘳(𝘴) 𝘢𝘯𝘥 𝘮𝘢𝘺 𝘯𝘰𝘵 𝘳𝘦𝘱𝘳𝘦𝘴𝘦𝘯𝘵 𝘮𝘺 𝘰𝘸𝘯 𝘱𝘦𝘳𝘴𝘰𝘯𝘢𝘭 𝘣𝘦𝘭𝘪𝘦𝘧𝘴/𝘷𝘪𝘦𝘸𝘴; 𝘐 𝘥𝘰 𝘯𝘰𝘵 𝘤𝘭𝘢𝘪𝘮 𝘵𝘰 𝘢𝘨𝘳𝘦𝘦 𝘰𝘳 𝘥𝘪𝘴𝘢𝘨𝘳𝘦𝘦 𝘸𝘪𝘵𝘩 𝘢𝘯𝘺 𝘰𝘧 𝘵𝘩𝘦 𝘤𝘰𝘯𝘵𝘦𝘯𝘵𝘴 𝘱𝘳𝘦𝘴𝘦𝘯𝘵𝘦𝘥. 𝘛𝘩𝘪𝘴 𝘤𝘰𝘯𝘵𝘦𝘯𝘵 𝘪𝘴 𝘯𝘰𝘵 𝘪𝘯𝘵𝘦𝘯𝘥𝘦𝘥 𝘢𝘴 𝘮𝘦𝘥𝘪𝘤𝘢𝘭 𝘰𝘳 𝘭𝘦𝘨𝘢𝘭 𝘢𝘥𝘷𝘪𝘤𝘦.
𝘔𝘺 𝘱𝘳𝘪𝘮𝘢𝘳𝘺 𝘨𝘰𝘢𝘭 𝘪𝘴 𝘵𝘰 𝘱𝘳𝘰𝘷𝘪𝘥𝘦 𝘢𝘤𝘤𝘦𝘴𝘴 𝘵𝘰 𝘪𝘯𝘧𝘰𝘳𝘮𝘢𝘵𝘪𝘰𝘯 𝘴𝘰 𝘰𝘵𝘩𝘦𝘳𝘴 𝘮𝘢𝘺 𝘤𝘰𝘮𝘦 𝘵𝘰 𝘵𝘩𝘦𝘪𝘳 𝘰𝘸𝘯, 𝘮𝘰𝘳𝘦 𝘥𝘪𝘷𝘦𝘳𝘴𝘦𝘭𝘺 𝘪𝘯𝘧𝘰𝘳𝘮𝘦𝘥, 𝘤𝘰𝘯𝘤𝘭𝘶𝘴𝘪𝘰𝘯𝘴.

𝘍𝘈𝘐𝘙-𝘜𝘚𝘌 𝘊𝘖𝘗𝘠𝘙𝘐𝘎𝘏𝘛 𝘋𝘐𝘚𝘊𝘓𝘈𝘐𝘔𝘌𝘙
*𝘊𝘰𝘱𝘺𝘳𝘪𝘨𝘩𝘵 𝘋𝘪𝘴𝘤𝘭𝘢𝘪𝘮𝘦𝘳 𝘜𝘯𝘥𝘦𝘳 𝘚𝘦𝘤𝘵𝘪𝘰𝘯 107 𝘰𝘧 𝘵𝘩𝘦 𝘊𝘰𝘱𝘺𝘳𝘪𝘨𝘩𝘵 𝘈𝘤𝘵 1976, 𝘢𝘭𝘭𝘰𝘸𝘢𝘯𝘤𝘦 𝘪𝘴 𝘮𝘢𝘥𝘦 𝘧𝘰𝘳 "𝘧𝘢𝘪𝘳 𝘶𝘴𝘦" 𝘧𝘰𝘳 𝘱𝘶𝘳𝘱𝘰𝘴𝘦𝘴 𝘴𝘶𝘤𝘩 𝘢𝘴 𝘤𝘳𝘪𝘵𝘪𝘤𝘪𝘴𝘮, 𝘤𝘰𝘮𝘮𝘦𝘯𝘵𝘪𝘯𝘨, 𝘯𝘦𝘸𝘴 𝘳𝘦𝘱𝘰𝘳𝘵𝘪𝘯𝘨, 𝘵𝘦𝘢𝘤𝘩𝘪𝘯𝘨, 𝘴𝘤𝘩𝘰𝘭𝘢𝘳𝘴𝘩𝘪𝘱, 𝘢𝘯𝘥 𝘳𝘦𝘴𝘦𝘢𝘳𝘤𝘩. 𝘍𝘢𝘪𝘳 𝘶𝘴𝘦 𝘪𝘴 𝘢 𝘶𝘴𝘦 𝘱𝘦𝘳𝘮𝘪𝘵𝘵𝘦𝘥 𝘣𝘺 𝘤𝘰𝘱𝘺𝘳𝘪𝘨𝘩𝘵 𝘴𝘵𝘢𝘵𝘶𝘵𝘦 𝘵𝘩𝘢𝘵 𝘮𝘪𝘨𝘩𝘵 𝘰𝘵𝘩𝘦𝘳𝘸𝘪𝘴𝘦 𝘣𝘦 𝘪𝘯𝘧𝘳𝘪𝘯𝘨𝘪𝘯𝘨. 𝘕𝘰𝘯-𝘱𝘳𝘰𝘧𝘪𝘵, 𝘦𝘥𝘶𝘤𝘢𝘵𝘪𝘰𝘯𝘢𝘭 𝘰𝘳 𝘱𝘦𝘳𝘴𝘰𝘯𝘢𝘭 𝘶𝘴𝘦 𝘵𝘪𝘱𝘴 𝘵𝘩𝘦 𝘣𝘢𝘭𝘢𝘯𝘤𝘦 𝘪𝘯 𝘧𝘢𝘷𝘰𝘳 𝘰𝘧 𝘧𝘢𝘪𝘳 𝘶𝘴𝘦.